Frog Virus 3 Deoxyribonucleic Acid

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Frog virus 3 deoxyribonucleic acid.

SUMMARY DNA extracted from frog virus 3 has a GC mol fraction of about 0"56. The mol. wt. of the DNA is about Ioo x I@ (97 × IO 6 by neutral sucrose gradient sedimentation; IO2 × IO 6 from renaturation kinetics). Analysis by neutral sucrose sedimentation indicated that the DNA exists as a single heteroduplex in the virus particle. Alkaline sucrose gradient sedimentation indicated that single st...

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Phosphonoacetic acid at concentrations above 200 mug/ml inhibited the replication of frog virus 3 in BHK cells. The inhibition of viral DNA replication observed in these cells was reversible and correlated with the inhibition of the virus-induced DNA polymerase activity in an in vitro assay. The synthesis of frog virus 3-induced late or gamma polypeptides was also inhibited by phosphonoacetic a...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 1974

ISSN: 0022-1317,1465-2099

DOI: 10.1099/0022-1317-24-2-339